In the world of laboratory equipment, the choice of a PCR thermal cycler can be instrumental in achieving precise experimental results. Two notable products in this field are the PCR Thermal Cyclers YR01868 by Kalstein and the Thermo Scientific™ PikoReal™ Real-Time PCR System from Thermo Fisher Scientific. These sophisticated machines play a crucial role in DNA amplification, pivotal for various genetic and microbiological studies.
The PCR Thermal Cyclers YR01868 from Kalstein boasts advanced temperature control and uniformity, ensuring consistent results across all samples. Its design is user-friendly, featuring an intuitive interface for ease of operation in high-throughput environments. A significant advantage of this model is its affordability, making high-end PCR technology accessible. In contrast, the Thermo Scientific™ PikoReal™ Real-Time PCR System offers real-time analysis with high sensitivity. However, it operates at a higher price point, which may not be suitable for all laboratories, particularly those with budget constraints.
Description of the Products
PCR Thermal Cyclers YR01868 is designed for high performance and reliability in genotyping, gene expression analysis, and genetic variation research. It features a gradient function, allowing simultaneous multiple tests with different annealing temperatures, enhancing efficiency. This model supports 96-well plate formats ideal for various lab-scale experiments, ensuring results with high fidelity. Additionally, its compact design is perfect for labs with limited space.
On the other hand, the Thermo Scientific™ PikoReal™ Real-Time PCR System facilitates real-time quantification and detection of nucleic acids. It supports five detection channels and is renowned for quick run times and high sensitivity. The compact instrument fits easily into crowded labs, although its operation cost can be higher due to consumables and required reagents. Its software integration helps streamline data management processes suited for complex analyses.
| Feature | Kalstein PCR Thermal Cyclers YR01868 | Thermo Scientific PikoReal System |
|---|---|---|
| Temperature Range | 4°C to 99°C | 4°C to 99°C |
| Compatibile Format | 96-well plates | Real-time 24- or 96-well |
| Gradient Capability | Yes | No |
| Channel Detection | N/A | Up to 5 channels |
| User Interface | Intuitive touch screen | Integrated software |
How They Work
The PCR Thermal Cyclers YR01868 operates by cycling through specific temperatures to denature DNA, anneal primers, and extend sequences, a key process in DNA amplification. With precise thermal control, it ensures efficient and accurate results, a necessity for any diagnostic or research laboratory.
In contrast, the Thermo Scientific™ PikoReal™ Real-Time PCR System allows for real-time monitoring of the amplification process through fluorescence. This technology enables users to quantify DNA, RNA, and cDNA strands with each cycle, providing immediate insights that facilitate complex genetic analyses.
What is it Used For
The PCR Thermal Cyclers YR01868 from Kalstein is essential for amplifying DNA sequences in fields such as forensic analysis, gene sequencing, and clinical diagnostics. Its gradient function allows it to perform multiple concurrent experiments, enhancing productivity and efficiency in research settings.
Types
PCR thermal cyclers come in various forms, including standard thermal cyclers and real-time thermal cyclers. Standard models, like the PCR Thermal Cyclers YR01868, focus on precise temperature cycles for DNA replication. Real-time models, such as the Thermo Scientific™ PikoReal™ Real-Time PCR System, provide quantitative data during amplification, catering to specific research needs requiring immediate analysis.
Market Price
The PCR Thermal Cyclers YR01868 by Kalstein is priced competitively, typically ranging from $5,000 to $10,000, depending on additional features or configurations. In contrast, the Thermo Scientific™ PikoReal™ System can reach upwards of $15,000 to $20,000, making it a premium choice for laboratories with larger budgets looking for real-time capabilities.
Frequently Asked Questions
What are PCR thermal cyclers used for?
PCR thermal cyclers are used to amplify DNA sequences, essential in various fields such as genetics, biology, and forensic science.
How does the gradient function in Kalstein’s cycler work?
The gradient function allows multiple tests with different annealing temperatures to be conducted simultaneously, optimizing experimental procedures.
Is the Kalstein PCR Thermal Cycler user-friendly for beginners?
Yes, the Kalstein PCR Thermal Cycler features an intuitive interface that simplifies use for new users while ensuring precision for experts.
Advantages and Disadvantages
The PCR Thermal Cyclers YR01868 by Kalstein offers several advantages, including cost-effectiveness, a user-friendly interface, and a crucial gradient function not available in some expensive alternatives. However, it lacks real-time monitoring, which is a significant feature of the Thermo Scientific™ PikoReal™ System. While the latter provides excellent real-time data, its higher cost and ongoing operational expenses can be a disadvantage for some environments.
Field Applications
In practical scenarios, the PCR Thermal Cyclers YR01868 are applied in DNA sequencing, genotyping, and various diagnostic processes. Its reliable temperature control ensures accuracy in clinical and research settings. Real-time PCR systems like the Thermo Scientific model excel in applications requiring immediate data, such as viral load analysis and gene expression studies.
Recommendations
To optimize the use of the PCR Thermal Cyclers YR01868, regular maintenance and calibration are recommended to maintain thermal accuracy. Additionally, leveraging Kalstein Plus facilitates ease of purchasing and obtaining quotes promptly, aiding smooth procurement processes for laboratories.
If you’re looking for a fusion of innovation and quality, you’ve come to the right place. At Kalstein, we offer the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece of equipment with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer, we bring science to life—it’s time to be part of our community. https://kalstein.pk/product/pcr-thermal-cyclers-yr01868/.